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tiation. We have employed the crypts from Lgr5‐EGFP‐IRES‐creERT2 reporter mouse line to grow Lgr5+ enteroids.10 In this article, we sum‐ marize the procedure of organoid culture derived from single Lgr5+ cells, and provide a simple way to examine their viability and functions. An overview of the experimental workflow is given in Figure 1.

Helicobacter pylori Activates and Expands Lgr5(+) Stem Cells View Lgr5 allele: origin, molecular description, gene associations, and references. Neonate (P1) Lgr5-EGFP-ires-CreERT2/R26RLacZ mice were given a single 100 μg dose of 4-hydroxytamoxifen to activate the Cre enzyme and, as a consequence, permanently switch on the lacZ reporter gene within renal Lgr5 +ve cells. Lgr5-EGFP-IRES-creERT2基因敲入工具鼠,产品简介：携带 Lgr5-EGFP-IRES-creERT2“基因敲入”的杂合小鼠，破坏了内源 Lgr5 基因功能，并表达了 EGFP 和 CreERT2 融合蛋白。当该品系与含 loxP 侧翼序列的小鼠杂交后，他莫昔芬诱导的 Cre 介导的重 To delete exons 2–10 of Trp53 in colonic stem cells, Trp53 flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-EGFP-IRES-creERT2 mice (Jackson, 008875; ref. 15). All mice were crossed onto a C57BL/6 background for at least 10 generations. Although small and large intestines possess seemingly similar Wnt-driven leucine-rich repeat-containing G protein–coupled receptor 5 (Lgr5)+ adult epithelial stem cells, we report here that the two organs exhibit distinct mechanisms of tissue response to ionizing radiation. Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007).

Lgr5-egfp-ires-creert2

An overview of the experimental workflow is given in Figure 1. Lineage tracing in the small intestine and colon.a, Lgr5-EGFP-IRES-creERT2 knock-in mouse crossed with Rosa26-lacZ reporter mice 12 h after tamoxifen injection. b, Frequency at which the blue Se hela listan på genoway.com ApcMin and Lgr5-EGFP-ires-CreERT2 mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Foxf1 and Foxf2 targeted mutants have been described elsewhere.22,23 Tg(FOXF2) was generated by pronuclear injection of a linearized BAC (RP4-668J24) spanning 120,429 base pairs of the human FOXF2 locus. 2020-06-10 · (n = 1 with 3 replicates for WT, all C3(1)-Tag, and healthy MMTV-PyMT; n = 2 MMTV-PyMT for Lgr4, Lgr6, and Lgr5 carcinoma, n = 4 MMTV-PyMT for Lgr5 hyperplasia and adenoma) b Tamoxifen (Tam)-induced Cre recombinase knocked into the Lgr5 locus, Lgr5-EGFP-IRES-creERT2 crossed to Tomato reporter mice, Ai14(R26R dtTomato) indicated Lgr5 + cell progeny in hyperplasias (whole mounts, 1-week Tam Lgr5EGFP-Ires-CreERT2/Apc fl/flmice with the R26R-Confetti strain. Upon stochastic Cre induction by low-dose tamoxifen in adult mice, intestinal ade-nomas developed from Apc-deleted Lgr5+ stem cells.After4weeks,weobservedlargeadenomas (fig. S2, A to C) that either uniformly expressed a single Confetti colororconsistedofanatomically To this end, we first established a sorting strategy that would enable the isolation of pure populations of Lgr5 + E10.5-E12.5 hepatoblasts using the Lgr5-EGFP-IRES-creERT2 mouse line, where the eGFP reporter is knocked-in into the Lgr5 locus (Barker et al., 2007), combined with co-staining using an anti-Liv2 antibody, which specifically labels E9.5-E13.5 liver progenitors (Nierhoff et al 2021-01-15 · Lgr5-eGFP-IRES-CreERT2 mice were crossed with B6.Cg-Gt (ROSA)26Sortm9(CAG-tdTomato)Hze/J mice (Jackson Laboratories) to generate Lgr5-eGFP-IRES-CreERT2; Rosa26-CAG-tdTomato heterozygote.

Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function

Lgr5. Lgr5 Gene Lgr5 Antibody. Hemsida. Expression of Lgr5 protein in the human endometrium.

To this end, we first established a sorting strategy that would enable the isolation of pure populations of Lgr5 + E10.5-E12.5 hepatoblasts using the Lgr5-EGFP-IRES-creERT2 mouse line, where the eGFP reporter is knocked-in into the Lgr5 locus (Barker et al., 2007), combined with co-staining using an anti-Liv2 antibody, which specifically labels E9.5-E13.5 liver progenitors (Nierhoff et al

Employing Lgr5-lacZ transgenic mice and Lgr5 in situ hybridization, we found colonic epithelial stem cells (CESC Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007). In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high). pressesLgr5-EGFPintheLgr5EGFP-Ires-CreERT2knock-inmice.The results showed that Lgr5-EGFP cells did not express the HBC markers, K14 and ICAM-1 (Fig. 1D,E).

creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of We then crossed the stem-cell-specific Lgr5-EGFP-IRES-creERT2 knockin mice to Apcflox/flox mice. In the resulting mice, Cre was. *These authors contributed Lgr5陽性細胞でGFPとCreリコンビナーゼタンパク質を発現するLgr5-EGFP-Ires- CreERT2トランスジェニックマウスを、通称レインボーマウスと呼ばれる While homozygous mice are not viable, heterozygous Lgr5-EGFP-IRES-CreERT2 mice are viable and fertile; harboring a Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 (Gpr49) gene function and expresses EGFP and CreERT2 fusion protein from the Lgr5 promoter/enhancer elements.
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Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function Jun 12, 2019 (B-H) Embryonic liver organoids were generated from sorted Lgr5-GFP+ hepatoblasts isolated from E10.5-E12.5 Lgr5-EGFP-IRES-creERT2 quence of adenoma formation, we generated Lgr5-EGFP-. IRES-creERT2; APCflox/flox mice. Intraperitoneal injection of tamoxifen induced acute loss of APC in a Lgr5-EGFP-IRES-. creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of created miR-34aflox/flox mice/Lgr5-GFP-IRES-CreERT2 mice. cells, we cultured mouse intestinal cells from Lgr5-EGFP-IRES-CreERT2 mice in 3D.

南模生物. 利用CRISPR基因编辑技术，将EGFP-IRES-creERT2-WPRE-polyA插入到小鼠Lgr5基因起始密码子处。 Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were treated with 12 Gy whole-body irradiation.
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Heterozygous mice harbor an Lgr5-EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function and expresses EGFP and CreERT2 fusion protein. When these mice mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the floxed sequences in the Lgr5-expressing cells of the offspring.

To induce the expression of the reporter gene, a single We crossed Lgr5-EGFP-IRES-CreERT2 knock-in and ROSA26-tdTomato (LRT) mice, and treated them with or without 4-hydroxytamoxifen (4-OHT), to determine Aug 1, 2012 To study the behavior of Lgr5+ cells within Apc-mutant adenomas, we then crossed Lgr5EGFP-Ires-CreERT2/Apcfl/fl mice with the R26R-Confetti Exogenous IFN-γ depletes Paneth cells and modifies the expression of rapidly cycling ISC markers. Heterozygous Lgr5 Egfp Ires Creert2 Mice, supplied by The Jan 15, 2021 Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of (C) MGs were isolated from Lgr5-EGFP mice and analyzed by flow cytometry for iments, Lgr5-EGFP-IRES-creERT2 mice were crossed into the LifeAct-RFP. Also Known As:Lgr5-EGFP-IRES-creERT2. Heterozygous mice harbor an Lgr5- EGFP-IRES-creERT2 "knock-in" allele that both abolishes Lgr5 gene function Jun 12, 2019 (B-H) Embryonic liver organoids were generated from sorted Lgr5-GFP+ hepatoblasts isolated from E10.5-E12.5 Lgr5-EGFP-IRES-creERT2 quence of adenoma formation, we generated Lgr5-EGFP-. IRES-creERT2; APCflox/flox mice.